abstract：:Catecholamines are known to have an inhibitory effect on oviductal smooth musculature by changing the adrenergic receptors activity. In order to further investigate the role of epinephrine and/or norepinephrine in oviduct, the distribution of beta-adrenergic receptors has been studied in the rat oviduct, using in vitr...

journal_title：Molecular and cellular endocrinology

authors： Tolszczuk M,Pelletier G

更新日期：1988-11-01 00:00:00

abstract：:Adenylate cyclase activity and its modulation by guanine nucleotides and isoproterenol were assessed in adipocyte membranes of mice with mutations causing different genetic obesity syndromes. The object was to determine whether the defect in inhibitory modulation observed in the obese (ob/ob) mouse was also present in...

journal_title：Molecular and cellular endocrinology

authors： Bégin-Heick N,Coleman DL

更新日期：1988-10-01 00:00:00

abstract：:The effects of 3.0 mM Mn2+ on parathyroid hormone (PTH) release, 45Ca fluxes and the cytoplasmic Ca2+ concentration (Ca2+i) were studied in vitro with use of parathyroid glands from rats and cattle. Mn2+ inhibited PTH release by about 70 and 60% at Ca2+ concentrations of less than 10 nM or 0.5 mM, respectively, and li...

journal_title：Molecular and cellular endocrinology

authors： Johansson H,Larsson R,Wallfelt C,Rastad J,Akerström G,Gylfe E

更新日期：1988-09-01 00:00:00

abstract：:C-fos promoter activity was examined in FRTL5 rat thyroid cells transiently transfected with a chimeric gene containing the c-fos promoter region linked upstream of the receptor gene chloroamphenicol acetyl transferase (CAT). Thyroid-stimulating hormone (TSH) stimulation of transfected cells increased CAT activity 2- ...

journal_title：Molecular and cellular endocrinology

authors： Damante G,Rapoport B

更新日期：1988-08-01 00:00:00

abstract：:Transforming growth factor beta (TGF beta) caused a dose-dependent increase in both basal and follicle-stimulating hormone (FSH)-stimulated inhibin production by rat granulosa cells in culture. The TGF beta dose-response curve in the absence of FSH was approximately parallel to that in the presence of either a minimal...

journal_title：Molecular and cellular endocrinology

authors： Zhang ZW,Findlay JK,Carson RS,Herington AC,Burger HG

更新日期：1988-08-01 00:00:00

abstract：:Pro-opiomelanocortin (POMC) is the common precursor of several pituitary hormones including alpha-melanotropic hormone, adrenocorticotropic hormone, beta-lipotropin and beta-endorphin. The porcine POMC cDNA was inserted downstream from the late promoter of an SV40-derived expression vector and co-transfected in NIH 3T...

journal_title：Molecular and cellular endocrinology

authors： Zollinger L,Noël G,Des Parois L,Sales V,Crine P,Boileau G

更新日期：1988-07-01 00:00:00

abstract：:The interaction of indomethacin with the antilipolytic effect of prostaglandin E2 (PGE2) was investigated in rat adipocytes in order to reveal a possible role of endogenous PGs in adipose tissue. Adipocytes isolated from rats treated in vivo for 5 days with indomethacin were compared with non-treated control rats. The...

journal_title：Molecular and cellular endocrinology

authors： Richelsen B

更新日期：1988-07-01 00:00:00

abstract：:The acquisition of steroidogenic competence by prothoracic glands of last instar Manduca sexta larvae is regulated by juvenile hormone (JH). Topical treatment of pre-commitment larvae with JH I or (7S)-hydroprene (a JH analog) delays development by increasing the time to pupal commitment and wandering. Prothoracic gla...

journal_title：Molecular and cellular endocrinology

authors： Watson RD,Bollenbacher WE

更新日期：1988-06-01 00:00:00

abstract：:It has recently been reported that phenol red, a pH indicator present in most tissue culture media, is a weak estrogen that can stimulate some estrogen-sensitive cells. However, the relative impact of phenol red on various cell lines is controversial. We examined the effect of phenol red on several estrogen-responsive...

journal_title：Molecular and cellular endocrinology

authors： Welshons WV,Wolf MF,Murphy CS,Jordan VC

更新日期：1988-06-01 00:00:00

abstract：:Rat adipocytes in primary culture have been used to study the intracellular processing of growth hormone (GH) receptors. Pretreatment of adipocytes with 20 micrograms/ml cycloheximide resulted in a rapid decline (t1/2 approximately 45 min) of the 125I-human growth hormone (hGH) binding capacity of the cells. This decl...

journal_title：Molecular and cellular endocrinology

authors： Roupas P,Herington AC

更新日期：1988-05-01 00:00:00

abstract：:A rapid and sensitive assay of iodothyronine-5'-monodeiodinase (5'-D) was developed using Sephadex column chromatography for separation of substrate 125I-rT3 from the product free 125I-. The distribution of 5'-D activity on rat kidney cortex cell membranes was examined in isopycnic zonal centrifugation experiments usi...

journal_title：Molecular and cellular endocrinology

authors： Boye N,Laurberg P,Jørgensen PL

更新日期：1988-03-01 00:00:00

abstract：:Using hybridization techniques and Northern blots we have identified a approximately 6.5 kb glucocorticoid receptor mRNA species in rat pituitary intermediate lobe. Glucocorticoid receptor mRNA concentrations, which are barely detectable or undetectable in normal animals, were greatly increased following ovariectomy. ...

journal_title：Molecular and cellular endocrinology

authors： Pfeiffer A,Barden N

更新日期：1988-02-01 00:00:00

abstract：:Oestrogens are known to exert specific stimulatory effects on basal and dopamine (DA)-inhibited prolactin (PRL) release as well as on PRL cell content in rat adenohypophysial cells in primary culture. Recently, we have demonstrated that classical oestrogens increase growth hormone (GH) release and cellular GH content ...

journal_title：Molecular and cellular endocrinology

authors： Simard J,Vincent A,Duchesne R,Labrie F

更新日期：1988-02-01 00:00:00

abstract：:Inhibitor-1 following phosphorylation by protein kinase A inhibits phosphoprotein phosphatase-1. We have found that in the rat heart inhibitor-1 is present only in the cytosolic fraction and that its phosphorylation in ventricular slices was increased by isoproterenol but not by isoproterenol and propranolol together....

journal_title：Molecular and cellular endocrinology

authors： Iyer RB,Koritz SB,Kirchberger MA

更新日期：1988-01-01 00:00:00

abstract：:The in vitro secretion of immunoreactive somatomedin C/insulin-like growth factor I (IR Sm-C/IGF-I) by two human breast cancer cell lines, MCF-7 and EVSA-T has been studied. IR Sm-C/IGF-I concentration showed a linear increase in serum-free culture media over 72 h of incubation for both cell lines, and a close correla...

journal_title：Molecular and cellular endocrinology

authors： Minuto F,Del Monte P,Barreca A,Nicolin A,Giordano G

更新日期：1987-12-01 00:00:00

abstract：:Inhibin production by cultured granulosa cells from immature diethylstilbestrol (DES)-primed rats was studied in relation to estradiol and progesterone production. The inhibin content in culture media was assayed with a specific radioimmunoassay (RIA) using an antibody to porcine 32 kDa inhibin that recognizes rat inh...

journal_title：Molecular and cellular endocrinology

authors： Suzuki T,Miyamoto K,Hasegawa Y,Abe Y,Ui M,Ibuki Y,Igarashi M

更新日期：1987-12-01 00:00:00

abstract：:Recently, the structures of two forms of inhibin present in human follicular fluid were elucidated from the corresponding cDNA sequences. Using specific antisera generated against the alpha-chain common to both forms, we have examined the cellular localization of inhibin in the male and female rat gonads and in human ...

journal_title：Molecular and cellular endocrinology

authors： Merchenthaler I,Culler MD,Petrusz P,Negro-Vilar A

更新日期：1987-12-01 00:00:00

abstract：:The synthesis and phosphorylation of H1 histones were studied in organ cultures of midpregnant mouse mammary glands exposed to various combinations of insulin, cortisol and prolactin over a 48-h period. The synthesis of specific H1 histone subtypes was changed only when all three hormones were present, and the effect ...

journal_title：Molecular and cellular endocrinology

authors： Hohmann P,Hughes C

更新日期：1987-11-01 00:00:00

abstract：:A new strain, named WRT cells, has been generated from primary cultures of rat thyroids. The primary culture was grown in Coon's modified Ham's F12 medium with 5% calf serum, insulin, hydrocortisone, transferrin, somatostatin, glycyl-L-histidyl-L-lysine and thyrotropin (TSH). On the basis of the following facts, the W...

journal_title：Molecular and cellular endocrinology

authors： Brandi ML,Rotella CM,Mavilia C,Franceschelli F,Tanini A,Toccafondi R

更新日期：1987-11-01 00:00:00

abstract：:The distribution and molecular forms of bombesin-like immunoreactivity (BLI) were determined in the ovine median eminence using a new, C-terminally directed antiserum. BLI was confined to neurons of the external zone of the median eminence, near small blood vessels, many of which also were immunoreactive for CRF. Each...

journal_title：Molecular and cellular endocrinology

authors： Giraud AS,Smith AI,Rundle SE

更新日期：1987-10-01 00:00:00

abstract：:The effect of a polyclonal anti-insulin receptor antibody (pIgG) on the insulin receptor tyrosine kinase (IRTK) activity toward poly-(Glu-Tyr) was examined using wheat germ agglutinin agarose-purified insulin receptors from rat liver membranes. The main effect of pIgG was a reduction of Vmax (from 60.8 to 31.8 pmol/mi...

journal_title：Molecular and cellular endocrinology

authors： Gherzi R,Cordera R,Andraghetti G,De Pirro R,Freidenberg GR,Lauro R,Adezati L

更新日期：1987-09-01 00:00:00

abstract：:The interaction between thyroid microsomal autoantibodies and thyroid microsomal antigen/thyroid peroxidase (TPO) has been studied using both intact antigen preparations and their water-soluble trypsin fragments. In an analysis of sera from 30 patients with Graves' or Hashimoto's diseases, microsomal antibodies showed...

journal_title：Molecular and cellular endocrinology

authors： Nakajima Y,Howells RD,Pegg C,Jones ED,Smith BR

更新日期：1987-09-01 00:00:00

abstract：:The binding of triiodothyronine (T3) to tadpole liver, intestine, and tail fin nuclei was consistent with a single class of binding sites. The KD for the binding of T3 to isolated nuclei from the liver (1.02 nM), intestine (1.40 nM), and tail fin (0.831 nM) nuclei were not significantly different. The number of bindin...

journal_title：Molecular and cellular endocrinology

authors： Jaffe RC

更新日期：1987-07-01 00:00:00

abstract：:We tested hamster uterine progesterone receptor (Rp) forms for binding to different chromatin preparations. Similar forms of chick oviduct Rp were used for comparison. Hamster Rp elutes from DEAE-Sephacel in the two peaks, peak I at 115 mM KCl and peak II at 205 mM KCl. Chick Rp peaks I and II elute at 125 mM and 300 ...

journal_title：Molecular and cellular endocrinology

authors： Cobb AD,Leavitt WW

更新日期：1987-07-01 00:00:00

abstract：:A peptide of unknown structure was found as a side product in a commercial preparation of the 1-34 fragment of bovine parathyroid hormone (PTH). CNBr cleavage and amino acid analysis showed that this peptide is the des-lys-13 form of 1-34 bovine PTH. The peptide thus represents a deletion mutant of PTH and structure-f...

journal_title：Molecular and cellular endocrinology

authors： Zull JE,Smith LM,Chuang J,Jentoft J

更新日期：1987-06-01 00:00:00

abstract：:An additional form of basic fibroblast growth factor (FGF), possessing full biological activity, has been isolated from bovine pituitary extracts. This polypeptide mitogen exhibits an apparent molecular weight of 17,500 by SDS-PAGE and lacks the first four N-terminal amino acid residues (Pro-Ala-Leu-Pro) of a previous...

journal_title：Molecular and cellular endocrinology

authors： Bertolini J,Hearn MT

更新日期：1987-06-01 00:00:00

abstract：:The effects of propylthiouracil (PTU) treatment on vasopressin, angiotensin II, glucagon and alpha 1-adrenergic receptors in both developing and adult rats were studied in liver membrane preparations by measuring the binding of the following ligands: [3H][8-lysine]vasopressin, [3H]Sar-angiotensin II, [125I]glucagon an...

journal_title：Molecular and cellular endocrinology

authors： Ali M,Cantau B,Chicot D,Clos J

更新日期：1987-05-01 00:00:00

abstract：:The binding of vasopressin, angiotensin II and prazosin (alpha 1-adrenergic antagonist) to purified heavy (GH) and (intermediate + light) (GI + L) rat liver Golgi fractions was studied. The three types of ligands showed a saturable and specific binding in Golgi fractions; the maximal specific binding of [3H]vasopressi...

journal_title：Molecular and cellular endocrinology

authors： Cantau B,Guillemette G,Chicot D,Devilliers G

更新日期：1987-05-01 00:00:00

abstract：:Renin secretion was stimulated in rats by adrenalectomy (ADX), by treatment with the converting enzyme inhibitor enalapril (MK 421), or by feeding a low salt diet plus furosemide treatment (FUR). Relative amounts of renin mRNA were measured by densitometric Northern blot analysis using a 32P-labeled 700 bp mouse subma...

journal_title：Molecular and cellular endocrinology

authors： Ludwig G,Ganten D,Murakami K,Fasching U,Hackenthal E

更新日期：1987-04-01 00:00:00

abstract：:Exposure of human decidual cells for 0.5 h to dibutyryl cAMP, isobutyl-methylxanthine (IBMX), cholera toxin or forskolin caused a dose-dependent inhibition of prolactin release with maximal inhibition by each agent of 50-60%. Dibutyryl cAMP (5 mM), IBMX (0.5 mM), and cholera toxin (10 micrograms/ml) also inhibited pro...

journal_title：Molecular and cellular endocrinology

authors： Handwerger S,Harman I,Costello A,Markoff E

更新日期：1987-03-01 00:00:00

abstract：:We studied the functional properties of hepatic glucagon receptors during rat development. Glucagon binding to liver membranes and isolated hepatocytes was significantly less in foetuses and weaning rats than in adult animals, reflecting changes in the number of receptors rather than any change in receptor affinity fo...

journal_title：Molecular and cellular endocrinology

authors： Blázquez E,Perez Castillo A,de Diego JG

更新日期：1987-02-01 00:00:00

abstract：:In the frog Xenopus laevis, vitellogenin is the major estrogen-induced protein in the liver. We have characterized an additional secreted protein, of 45,000 Da and designated Ep45, which is also responsive to estrogen treatment. Like vitellogenin, Ep45 is not normally found in the plasma nor synthesized by the liver o...

journal_title：Molecular and cellular endocrinology

authors： Holland LJ,Wangh LJ

更新日期：1987-01-01 00:00:00

abstract：:The aim of this work was to determine whether treatments of rats with estradiol (E) in conditions known to decrease the proliferation rate, the mitotic index and the thymidine incorporation into the DNA of the MtTF4 tumor act at a specific point in the cell cycle. Two weeks after grafting a piece of tumor under the ki...

journal_title：Molecular and cellular endocrinology

authors： Pharaboz MO,Cordier G,André J

更新日期：1986-12-01 00:00:00

abstract：:Micropipettes were used to record electrical activity from single neurons in hypothalamic tissue slices, in the hypothalamic arcuate nucleus (ARC), and in the periventricular and suprachiasmatic preoptic nuclei (POA). Responses were measured following in vitro application of luteinizing hormone releasing hormone (LHRH...

journal_title：Molecular and cellular endocrinology

authors： Pan JT,Kow LM,Kendall DA,Kaiser ET,Pfaff DW

更新日期：1986-12-01 00:00:00

abstract：:Octopamine serves as a neurotransmitter in the glandular lobe of the locust corpus cardiacum where it regulates adipokinetic hormone (AKH) secretion from intrinsic neurosecretory cells. Two AKHs (AKH I and II) from the corpus cardiacum of Locusta have been sequenced and synthesized. We have now demonstrated that octop...

journal_title：Molecular and cellular endocrinology

authors： Pannabecker T,Orchard I

更新日期：1986-12-01 00:00:00

abstract：:The Ca2+ dependency of the direct stimulatory effect of the gonadotropin-releasing hormone (GnRH) agonist analog [D-Ser(t-Bu)6]des-Gly10-GnRH-N-ethylamide (GnRHa) on progesterone production was investigated and compared to that of luteinizing hormone (LH) in rat granulosa cells from preovulatory follicles. Removal of ...

journal_title：Molecular and cellular endocrinology

authors： Eckstein N,Eshel A,Eli Y,Ayalon D,Naor Z

更新日期：1986-09-01 00:00:00

abstract：:Expression of the calcitonin (CT)/calcitonin gene related peptide (CGRP) gene and the proopiomelanocortin (POMC) gene has been demonstrated by Northern blot hybridization analysis of RNA extracted from human medullary thyroid carcinoma (MTC), pheochromocytoma and lung carcinoma. CT mRNA in these tumors could not be di...

journal_title：Molecular and cellular endocrinology

authors： Höppener JW,Steenbergh PH,Moonen PJ,Wagenaar SS,Jansz HS,Lips CJ

更新日期：1986-09-01 00:00:00

abstract：:The effect of insulin on human red blood cells was investigated, both on intact cells and on isolated plasma membranes, testing the responsiveness of membrane-bound enzymes--such as (Na+-K+)-ATPase and 5'-nucleotidase--as well as the ouabain binding and ionic fluxes. It appears that insulin stimulates Na-pumping mecha...

journal_title：Molecular and cellular endocrinology

authors： Baldini P,Incerpi S,Pascale E,Rinaldi C,Verna R,Luly P

更新日期：1986-07-01 00:00:00

abstract：:The growth of ZR-75-1 cells, a line of human breast cancer cells in culture, is stimulated by oestradiol and inhibited by anti-oestrogens. Changes in growth rate caused by these agents are accompanied by changes in activity of ornithine decarboxylase, a rate-limiting enzyme for polyamine synthesis. Furthermore, the gr...

journal_title：Molecular and cellular endocrinology

authors： Hoggard N,Green CD

更新日期：1986-06-01 00:00:00

abstract：:Autodigestion of nuclei isolated from the androgen-dependent Shionogi mouse mammary carcinoma revealed the presence of endogenous endonuclease activity which was stimulated by Ca2+ and Mg2+ and generated a repeating pattern of DNA fragments with an average monomeric size of 179 base pairs. The nuclear concentration of...

journal_title：Molecular and cellular endocrinology

authors： Giles MG,Rennie PS,Bruchovsky N

更新日期：1986-05-01 00:00:00